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已往数年中,我们取清华大学医学体系生物学研究中心协作,完成了包孕神经元监测芯片、细胞阻抗芯片、细胞电穿孔芯片、细胞支配芯片等在内的数十种微电极类芯片的设想、加工和测试,借完成了包孕毛细管电泳芯片、精子康健评价芯片、单细胞捕捉芯片、基因扩增和检测芯片等在内的数十种微流体类芯片的设想、加工和测试。相干研讨论文宣布正在Lab on a chip, Biosensors and Bioelectronics等国际有名杂志。

电阻抗芯片| 电扭转芯片| 神经细胞网络电心理芯片(MEA)| 体外授精芯片| 别的芯片展现|

 

电阻抗芯片什物图澳门葡京赌场官网研讨四种细胞迁徙速度

                               电阻抗芯片什物图                     插指阵列式设想及时监测细胞迁徙                  研讨四种细胞迁徙速度

     细胞电阻抗传感手艺是一种可以或许对细胞的生物学行动停止及时、定量、非符号和自动化检测的细胞剖析手艺。该手艺为细胞生物学研讨供应了一种奇特的视察平台,借助细胞电阻抗手艺,以静态视角正在细胞程度定量的研讨生命征象。芯片接纳微加工手艺制备而成,个中包孕磁控溅射、SU-8光刻、PDMS浇注、划片、压焊、等离子键合等多种工艺。

代表论文

[1] Wang L., Wang L., Yin H., Xing W., Yu Z., Guo M. and Cheng J.,“Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip”  Biosensors and Bioelectronics 2010,25:990-995.

[2] Wang L., Zhu J., Deng C., Xing W. and Cheng J.“An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing”Lab on a chip 2008,8:872-878.

[3] Wang L., Wang H., Wang L., Mitchelson K., Yu Z. and Cheng J.“Analysis of the sensitivity and frequency characteristics of coplanar electrical cell-substrate impedance sensors”Biosensors and Bioelectronics 2008,24:14-21.


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   电扭转芯片真图

     细胞生机电扭转检测芯片的道理是,当细胞被置于一个由外加高频交流电所发生的扭转电场中,会受迁移转变力矩的感化而迁移转变。当细胞心理状况不同时,其扭转状况也不 同。经由过程视察细胞正在芯片上扭转状况,便能够相识细胞的心理状况。属于非侵入式、非符号、无毁伤的检测体式格局,能够实现细胞活性的及时在线检测。芯片接纳微加 工手艺制备而成,个中包孕磁控溅射、光刻、湿法侵蚀、划片、压焊、热压等多种工艺。

代表论文

[1] Wang L., Guo M., Huang C. and Cheng J., "Continuous cell separation by chip-based travelling-wave dielectrophoresis and laminar flow", microTAS 2003, October 5-9, 2003, Squaw Valley, California USA.

[2] Huang C., Chen A., Wang L., Guo M. and Yu J., "Electrokinetic measurements of dielectric properties of membrane for apoptotic HL-60 cells on chip-based device", Biomedical Microdevices 2007, 9:335-343.

 


 

 


 

微电极阵列芯片构造展现  

微电极阵列芯片构造展现 

     该芯片接纳微电极阵列,正在体外对神经细胞的电心理旌旗灯号停止全自动、多通道、及时、一连监测。此类芯片是神经网络研讨的有力东西,有助于探究神经元群体信息传 递和贮存的历程,展现电运动到场大脑的生长发育、进修影象、觉得活动整合等历程的相干机制。该芯片接纳磁控溅射、PECVD、光刻、湿法侵蚀、干法刻蚀、 划片、压焊、等多种微加工工艺制备而成。

代表论文

[1] Pan L., Xiang G., Huang L., Yu Z., Cheng J., Xing W. and Zhou Y., "Automatic positioning and sensing microelectrode array (APSMEA) for multi-site electrophysiological recordings", Joumal of Neuroscience Methods 2008, 170:123-129.

[2] Xiang G., Pan L., Xing W., Zhang L., Huang L., Yu J., Zhang R., Wu J., Cheng J. and Zhou Y., "Indentification of activity-dependent gene expression profiles reveals specific subsets of genes induced by different routes of Ca2+ entry in cultured rat cortical neurons", Joumal of Cellular Physiology 2007, 212:126-136.

[3] Xiang G., Pan L., Huang L., Yu Z., Song X., Cheng J., Xing W. and Zhou Y., "Microelectrode array –based system for ordered neuropharmacological applications with cortical neurons cultured in vitro", Biosensors and Bioelectronics 2007, 22:2478-2484.

 


 


  体外受精芯片细节展现

体外授精芯片细节展现

     我们正在微流控芯片上设想微坑阵列和围栏阵列两种构造,胜利实现了卵细胞授精、受精卵的一连换液造就和晚期胚胎的获得。体外授精芯片经由过程微加工工艺加工而成,其重要要害工艺在于深邃比的SU-8模具加工,经由过程SU-8光刻工艺的革新,深邃宽比、优越高低垂直度的SU-8模具加工手艺已成为我们的通例工艺。

代表论文

[1] Xie L., Ma R., Han C., Su K., Zhang Q., Qiu T., Wang L., Huang G., Qiao J., Wang J. and Cheng J., "Integration of sperm motility and chemotaxis screening with a microchannel-based device", Clinical Chemistry 2010, 56:1270-1278.

[2] Han C., Zhang Q., Ma R., Xie L., Qiu T., Wang L., Mitchelson K., Wang J., Huang G., Qiao J., Cheng J., "Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device", Lab on a Chip 2010, 10:2848-2854.

[3] Ma R., Xie L., Han C., Su K., Qiu T., Wang L., Huang G., Xing W., Qiao J., Wang J. and Cheng J., "In vitro fertilization on a single-oocyte positioning system integrated with motile sperm selection and early embryo development", Analytical Chemistry 2011, 83:2964-2970.

 


 

 

 

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